Description and genomic characterization of Cohnella amylolytica sp. nov., isolated from Hot Springs in Yunnan province, south-west China

Abstract A bacterial strain, Gram staining positive, strictly aerobic, rod-shaped, motile bacterium with flagellum and endospore-forming, designated strain YIM B05605 T , was isolated from soil sampled in Hamazui hot springs, Tengchong City, Yunnan province, China. Optimum growth for the strain occurred at pH 7.0 and 45 °C. The predominant menaquinone of strain YIM B05605 T was MK-7. The diagnostic diamino acid in the cell-wall peptidoglycan was meso -diaminopimelic acid. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), unidentified glycolipid (GL), three unknown aminophospholipids (APLs) and unidentified polarlipid (PL) were part of the polar lipid profle. The major fatty acids were anteiso- C 15:0 and iso- C 16:0 . The DNA G+C content of the type strain was 58.76 mol%. Genome-based phylogenetic analysis confirmed that strain YIM B05605 T formed a distinct phylogenetic cluster within the genus Cohnella . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain YIM B05605 T with the most related species C. fontinalis YT-1101 T were 73.42 % and 15.7 %. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strain YIM B05605 T could be classified as a novel species of the genus Cohnella , for which the name Cohnella amylolytica sp. nov., is proposed. The type strain is YIM B05605 T (= CGMCC 1.60052 T = KCTC 43462 T = NBRC 115921 T ).