Abnormal homeostasis of P53 gene knockout mice can be reflected in urinary proteome

Abstract In this experiment, the urinary proteome analysis of p53 knockout mice at four relatively early time points before death was performed from three dimensions. One was to compare the differential proteins screened by homozygote and heterozygote at the same time point; the other was to compare the differential proteins screened by homozygote and wild-type as well as heterozygote and wild-type at the same time point; the third was to compare the differential proteins screened by homozygote and heterozygote before and after each time point, and to screen the differential proteins of three mice by self-control analysis and screening. Later, enrichment analysis of the differential proteins was performed mainly by Metascape and String, and disease association analysis of gene or variant genotype-phenotype with the help of DisGeNET and Monarch. We found that the biological pathways mainly related to metabolism were most abundant, and at the same time abnormal homeostasis, cancer and tumor, cardiovascular diseases, and neurodegenerative diseases appeared. However, under the analysis of Monarch, homozygotes or heterozygotes contained abnormal homeostasis at all time points, and most of them ranked the first or at least the top, while all timepoints in the growth and development of each normal rat were not enriched to abnormal homeostasis and other disease pathways.