Supplementary Figure 4 from Preexisting MEK1 Exon 3 Mutations in ^V600E/KBRAF Melanomas Do Not Confer Resistance to BRAF Inhibitors

PDF file - 76K, Relative expression levels of WT vs. P124SMEK1 in the YUKSI B-RAF/MEK1 double-mutant melanoma short-term culture. Genomic DNA (gDNA) and cDNA derived from YUKSI were subjected to exon 3 sequencing by the Sanger method. Relative peaks of WT vs. P124SMEK1 is shown to be 1:1 for both gDNA and cDNA based on nucleotide chromatograms, showing no allelic bias in RNA expression.