Antioxidative and anti‐ageing effects of nucleotide‐rich yeast hydrolysate by modulating metabolites in Caenorhabditis elegans based on metabonomic analysis

Summary In the food industry, yeast was used to regulate food flavour and produce fermented products. In recent years, research has found that yeast contains amino acids, peptides and other active substances, but there was little research on its improvement of health benefits. In this study, the antioxidant and anti‐ageing activities of nucleotide‐rich yeast hydrolysates (NYH) were explored in Caenorhabditis elegans nematode model system, it was found that the lifespan, anti‐heat stress and anti‐acute oxidative stress were enhanced in C. elegans supplemented with NYH by up‐regulating the activity of antioxidant enzymes and down‐regulating the level of MDA and ROS. The mRNA expression level such as age‐1 and daf‐2 was significantly down‐regulated. In addition, the antioxidant and anti‐ageing activities of NYH were exerted mainly through tyrosine metabolism, glycerophospholipid metabolism and glutathione metabolism metabolic pathways. These results showed that NYH had the potential of antioxidation and anti‐ageing, which provided a possibility for the development of yeast products as a functional food in food science.