A pyrF -Based Efficient Genetic Manipulation Platform in Acinetobacter baumannii To Explore the Vital DNA Components of Adaptive Immunity for I-F CRISPR-Cas

Shaofang Wu,Run Xu,Mengjiao Su,Can Gao, Yang Liu, Yujia Chen,Guangxin Luan, Jin Xu,Rui Wang

Microbiology spectrum（2022）

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摘要

In this study, we developed the widely applicable and efficient pyrF -based selection and counterselection system in A. baumannii for gene manipulation. In most cases, this pyrF /5-FOA genetic manipulation system was very effective and enabled us to obtain marker-free mutants in a very short period of time.

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关键词

acinetobacter baumannii,efficient genetic manipulation platform,dna,adaptive immunity,crispr-cas

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