The Protective Effect of the Experimental TiF4 and Chitosan Toothpaste on Erosive Tooth Wear in Vitro

Abstract This study evaluated the protective effect of an TiF 4 and chitosan toothpaste on erosive tooth wear (ETW) in vitro . Bovine samples were randomly assigned to toothpastes (n=12): 1) TiF 4 (1400 ppm F − ), 2) 0.5%. chitosan (75% deacetylation, 500 mPas), 3) TiF 4 (1400 ppm F − ) plus 0.5%. chitosan (75% deacetylation, 500 mPas), 4) Placebo, 5) Erosion Protection (Elmex® - GABA, Suíça, 1400 ppm F − ). Twelve samples were only eroded. All samples were submitted to erosive pH cycles and the groups 1-5 to abrasive challenges using toothpastes’ slurries plus 45 s of treatment, for 7 days. The final profile was overlaid to the baseline one for the ETW calculation (µm). The data were subjected to Kruskal-Wallis/Dunn tests. TiF 4 toothpastes, regardless of the presence of chitosan, were able to significantly reduce ETW compared to placebo, while chitosan alone was similar to placebo for both tissues. The toothpastes containing TiF 4 were even superior to the commercial Elmex® toothpaste on enamel wear, which were also significantly different from placebo. TiF 4 and Elmex® toothpastes minimized the impact of brushing on eroded surface compared to the other groups. In conclusion, TiF 4 toothpastes, regardless the presence of chitosan, showed to be effective in minimizing ETW in vitro .