Front Cover: Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA (ChemBioChem 21/2022)

A simple and versatile strategy is introduced for the selective fluorescent labeling of intercellular contacting cells. A transpeptidase sortase A and its substrate triglycine peptide (GGGYC sequence) were chemically conjugated with a lipidated polymer and then anchored onto the membranes of different cells. The triglycine modified cells (displayed in red) were labeled with a fluorescent dye by contacting with the sortase A-modified cells based on proximity-dependent transpeptidation with a dye-modified labeling peptide (DLPETGG sequence). More information can be found in the Research Article by S. Yamaguchi, A. Okamoto et al.