Effect of Lncrna FER1L4 Overexpression on Prognosis and Cell Proliferation in Non-Small Cell Lung Cancer

Abstract Background: The purpose of this study was to investigate the clinical significance and biological function of lncRNA FER1L4 in NSCLC. Methods : A total of 114 cases of NSCLC tissues and matched normal lung tissues were obtained from The first hospital of Jilin University between January 2009 and December 2016. The clinicopathological characteristics of patients were collected, including age, gender, tumor size, etc. The expression levels of LncRNA FER1L4 were detected by qRT-PCR. Non-small cell lung cancer cell lines (A549, H292 and H1299) were cultured with LncRNA FER1L4 mimics and LncRNA FER1L4 inhibitor. Cell proliferation was evaluated by CCK-8 and colony formation assay. Results : The expression levels of lncRNA FER1L4 were significantly lower in NSCLC tissues compared with those in matched normal lung tissues ( P <0.05). Decreased level of lncRNA FER1L4 was significantly correlated with tumor size, TNM stage and lymph node metastasis ( P <0.05). Moreover, lncRNA FER1L4 over-expression predicted favorable prognosis in NSCLC ( P <0.05). Furthermore, CCK-8 and colony formation assay showed that lncRNA FER1L4 over-expression significantly suppressed cell proliferation. Conclusions : Our results suggested that LncRNA FER1L4 overexpression predicted favorable prognosis and suppressed cell proliferation in NSCLC, which may serve as a promising therapeutic target.