Antibiogram assay of E.coli isolated from pus samples at lady reading hospital Peshawar

Background: Pyogenic infections are caused by both aerobic and anaerobic bacteria, which are widespread in the hospital setting and result in severe morbidity, extended hospitalization and a massive financial burden. Objective: To determine the antbiogram assay of E.coli isolated from pus samples. Methodology: This was prospective cross sectional study conducted at the Diagnostic Laboratory Lady Reading Hospital, Peshawar for duration of one year from November 2020 to November 2021. From various patients, a total of 365 pus samples were obtained. At 37°C, samples were incubated aerobically for 24 hours after being inoculated on MacConkey agar. The pus isolates were tested for antimicrobial susceptibility using the Kirby-Bauer disk diffusion technique, as recommended by the Clinical and Laboratory Standards Institute, and Mueller Hinton agar was used for antibiogram assay.  Results: Out of 365 pus samples E.coli was isolated from 36 (9.86%) samples. Amikacin and tobramycin were 100% sensitive to E.coli while it was 100% resistant to Cefuraxime and cephradine. Conclusion: Our study concludes that that the most effective antibiotics for E.coli in pus samples are Amikacin and tobramycin. Our research proposes that regular antibiotic susceptibility testing be performed for the purpose of determining appropriate empirical medication treatment and management.