Halomonas salipaludis sp. nov., isolated from the saline-alkali wetland soil

Abstract Strain WRN001 T , a Gram–staining–negative, strictly aerobic, non–motile bacterium was isolated from the natural saline-alkali wetland soil of Binhai new district, Tianjin, China (38°46′N, 117°13′E). Cells of strain WRN001 T were 0.3-0.5 µm in width and 1.5-2.5 µm in length, and the growth occurred optimally at 33-37 °C, pH 7.5-8.0, and in the presence of 8-10% (w/v) NaCl. Based on 16S rRNA gene sequence analysis, the isolate could be affiliated to the genus Halomonas , and the highest 16S rRNA gene sequence similarity of strain WRN001 T to its closest relative Halomonas qiaohouensis YIM QH88 T was 97.47%. The size of the genome as presented here was 5,475,884 bp with a G+C content of 63.8 mol %. The major respiratory quinone of strainWRN001 T was Q-9, and the dominant fatty acids were summed feature 8, summed feature 3, C 10:0 , C 12:0 , C 12:0 3-OH, C 16:0 , and C 17:0 cyclo. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phophatidylcholine (PC), two phospholipids (PL), aminolipid (AL), and three unidentified lipids (L). These data mentioned above combined with the low DDH values between strain WRN001 T and the close relative, Halomonas alkalitolerans 1513 T (42.20%) and base on comparisons with currently available genomes, the highest average nucleotide identity (ANIm) value was 91.39% to Halomonas alkalitolerans 1513 T (GenBank accession No. GCA_001971685.1). Therefore, we propose a novel species in the genus Halomonas to accommodate this novel isolate: Halomonas salipaludis sp. nov. (type strain WRN001 T = KCTC 52853 T = ACCC 19974 T ).