Metastatik Akciğer Kanserli Hastaların Periferik Kanında Yeni Aday Biyomarker: Yüksek DICER1 Ekspresyon Düzeyi

Objective: Lung cancer is in the first place in the world in terms of cancerrelated incidence and mortality. Since cancer is a multifactorial disease, it is very important to try to illuminate the mechanisms associated with cancer. It is known that miRNA activity, which is closely related to carcinogenesis, is impaired with various mechanisms. One of these mechanisms is the molecular changes that occur during miRNA process. DICER1 has an important function in microRNA biogenesis. In our study, it was aimed to investigate the effect of DICER1 gene expression level on metastatic lung cancer development. Material and Method: The study groups consist of peripheral blood samples of 47 patients with metastatic lung cancer who had recently been diagnosed but had never been treated and 45 healthy people. The investigated DICER1 target gene was examined simultaneously with the HPRT1 reference gene by using the Real-Time PCR method. The results of DICER1 gene expression levels of patients and those of healthy individuals were compared. The results were evaluated by using the 2-ΔΔCT method. Results: The expression level of DICER1 gene in the peripheral blood lymphocytes of patients with metastatic lung cancer was increased by 1.756 (2-ΔΔCt) fold compared to the healthy control group and the increased expression level was statistically significant, p <0.05. Discussion and Conclusion: The results of our study were found correlated with the results of the study on tumor tissue of lung cancer in the literature. In this context, it is suggested that DICER1 expression in the peripheral blood lymphocytes will provide information about the level of DICER1 expression in tumor tissue of metastatic lung patients whose tissues cannot be reached. In addition, the study is the first to investigate DICER1 expression in the peripheral blood samples of lung cancer patients.