Comparison of ivf outcomes from use of 2 different pgt-a laboratories

To compare clinical outcomes following PGT-A using two reference laboratories with different testing platforms. This retrospective study included all PGT-A cases from a single IVF center and embryology laboratory following the practice’s standard procedures. PGT Lab 1 was used between June 2015 and June 2022, and PGT Lab 2 was used between April 2022 and April 2023. Sustained implantation rate was determined based on observed gestational sacs, as well as presence of fetal heart beat and number of embryos transferred. Fisher’s Exact test was computed for statistical analysis of rates of no result, aneuploidy, biochemical pregnancy, clinical pregnancy, clinical loss, and sustained implantation. Student’s T test was computed to compare maternal age. A total of 266 PGT cases (1059 embryos) were tested by PGT Lab 1 and 97 PGT cases (350 embryos) were tested by PGT Lab 2. The average maternal age of PGT cases was similar (PGT Lab 1 = 37.8 years ± 4.4 (SD), PGT Lab 2 = 38.2 years ± 4.1 (SD), p=0.4). The rate of embryos with non-actionable results (no DNA amplification or inconclusive) was also similar (PGT Lab 1= 5.0%, PGT Lab 2 = 3.7%, p=0.4). The rate of aneuploid embryos was significantly higher in PGT Lab 1 (64.2%) vs PGT Lab 2 (48.0%) (p<0.00001). Table 1 summarizes clinical outcomes after transfer of euploid embryos tested by both PGT Labs. The rate of biochemical pregnancies did not differ between both groups. However, the sustained implantation rates and clinical pregnancy rates were significantly higher for embryos tested by PGT Lab 2. Despite significantly different aneuploidy rates detected by each lab, the rate of clinical pregnancy loss was not different. Overall, sustained implantation and clinical pregnancy rates were higher for embryos tested by PGT Lab 2. Although the embryo aneuploidy rate was significantly lower for PGT lab 2, average maternal age and the clinical loss rate between both PGT labs was comparable.