Sven Turkalj,1,2,4,6,* Niels Asger Jakobsen,1,2,3,4 Angus Groom,1,2,5 Felix A. Radtke,1,2,5 and Paresh Vyas1,2,3,7,*
STAR PROTOCOLS(2023)
摘要
Single-cell assay for transposase-accessible chromatin with sequencing (scATAC-seq) resolves the heterogeneity of epigenetic states across cells but does not typically capture exonic mutations, which limits our knowledge of how somatic mutations alter chromatin landscapes. Here, we present a plate-based approach coupling high-sensitivity genotyping of genomic loci with high-content scATAC-seq libraries from the same single cells. We first describe steps for optimization of genotyping primers, followed by detailed guidance on the preparation of both scATAC-seq and single-cell genotyping libraries, fully automated on high -throughput liquid handling platforms.For complete details on the use and execution of this protocol, please refer to Turkalj, Jakobsen et al.1
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要