Potential Antioxidant Activity of Novel Antioxidant Peptides from Protein Hydrolysate of Peony Seed Dreg in Chemical and H2O2-Induced RAW264.7 Cells
JOURNAL OF FOOD BIOCHEMISTRY(2023)
摘要
Peony seed dreg protein (PSDP) was hydrolyzed by proteases secreted by Pseudoalteromonas sp. CSN423. This hydrolysate exhibited excellent antioxidant capability after passing through a 3 kDa ultrafiltration membrane. The antioxidant peptides further purified by size-exclusion chromatography scavenged 2, 2-diphenyl-1-picrylhydrazyl (DPPHcenter dot ) (73.0 +/- 1.46%) and hydroxyl radicals (center dot OH) (81.17 +/- 3.69%), protected DNA from oxidative injury, and displayed an oxygen radical absorbance capacity (ORAC) of 4.04 +/- 1.11 mu mol TE/mg. Furthermore, the antioxidant peptide inhibited apoptosis by inhibiting the production of reactive oxygen species (ROS) and the decrease of mitochondrial membrane potential in H2O2-induced RAW264.7 cells. We identified four major peptides such as YFPF, ECCASLAPL, YVSLK, and YFEM by using mass spectrometry. YFPF showed a potent center dot OH scavenging capability of 75.30 +/- 1.08%. ECCASLAPL exhibited higher DPPHcenter dot scavenging capability (87.20 +/- 1.03%) than glutathione (GSH) (49.50 +/- 1.50%). Moreover, the peptide YVSLK exhibited the highest ORAC of 2.52 +/- 1.20 mu mol TE/mg of the identified peptides. This work highlights that the peptides from PSDP might have potential uses in cosmetics, functional foods, and drug development.
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