Contamination of commercial dry dog food by Aspergillus flavus and aflatoxins in Aguascalientes, Mexico
REVISTA MEXICANA DE CIENCIAS PECUARIAS(2023)
摘要
Commercial dry food (CDF) for dogs is a whole grain ration thoroughly mixed and die-cut with heat and pressure to give it the shape of kibble. CDF is formulated with several agro-industrial ingredients and by-products of agricultural and livestock origin. Contamination by Aspergillus flavus and aflatoxins (AFs) in foods has been shown to be a global problem that causes harm to human and animal health. The objective was to evaluate the presence of fungal microbiota and contamination by AFs in CDF. A random sample (n= 77) of marketed CDF was selected in Aguascalientes, Mexico. The samples were processed and cultured by serial dilutions, obtaining monosporic isolates, which were characterized morphologically, toxigenically (HPLC), and molecularly (PCR). The concentration of AFs in CDF was quantified by HPLC. Fungal growth was observed in 53.2 % of CDF, and 7.8 % exceeded the maximum permissible limit (MPL=10(6) CFU/g). The genera Aspergillus, Penicillium, Cladosporium, Mucor, Alternaria, and Fusarium were found (69.4, 12.9, 9.4, 4.7, 1.7, and 1.1 %, respectively). All CDF samples showed contamination by AFs (14.8 +/- 0.3 mu g/kg), and 11.8 % exceeded the MPL (20.0 mu g/kg) suggested by the regulations; contamination was significantly associated (P<0.05) with some ingredients used, CDF moisture, and inclusion of fungicides and sequestrants. The results obtained suggest that the CDF manufacturing process does not wholly eliminate contamination by fungi or by the AFs present in the ingredients used for its formulation; consequently, these remain in the finished product, putting at risk the health of dogs and the efficacy of the food chain.
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关键词
Aspergillus flavus,Aflatoxins,Food chain,Kibble
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