Chryseobacterium pyrolae sp. nov., isolated from the rhizosphere soil of Pyrola calliantha H.

A novel Gram- stain-negative, aerobic, non-motile, rod-shaped bacterium, designated pc2- 12T, was isolated from the rhizosphere soil of the herb Pyrola calliantha collected from arid areas of Tibet. The strain grew most vigorously with 1 % (w/v) NaCl, at pH 7.0 and at 25 degrees C. According to the results of 16S rRNA gene sequence analysis, pc2-12T was closely related to the members of the genus Chryseobacterium, with highest levels of sequence similarity to Chryseobacterium viscerum 687B-08T (98.42 %), Chryseobacterium oncorhynchi 701B-08T (98.11 %) and Chryseobacterium ureilyticum DSM 18017T (97.98 %). The average nucleotide identity values between pc2- 12T and C. viscerum 687B-08T, C. oncorhynchi 701B-08T and C. ureilyticum DSM 18017T were 79.71, 79.49 and 79.26 %, respectively. The in silico DNA-DNA hybridisation values between pc2- 12T and C. viscerum 687B-08T, C. oncorhynchi 701B-08T and C. ureilyticum DSM 18017T were 23.30, 23.00 and 22.90 %, respectively. The draft genome sequence of pc2- 12T was 4.64 Mb long, with DNA G+C content of 37.0 mol%. The fatty acids contained in the cells of pc2- 12T were mainly composed of iso-C15:0, iso-C17: 0 3 -OH and summed feature 3 (C16:1 omega 6c and/or C16:1 omega 7c). The main polar lipid was phosphatidylethanolamine. MK -6 was the sole respiratory quinone. On the basis of the results of analysis of all the data described, pc2- 12T is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium pyrolae sp. nov., is proposed. The type strain is pc2- 12T (=GDMCC 1.3256T= JCM 35712T).