Transposons Tn10 and Tn5
ASM Press eBooks(2015)
Abstract
Tn 10 and Tn5 are composite bacterial transposons (Fig. 1). Both these transposons, as well as their respective IS elements (IS10 and IS50), transpose by a nonreplicative cut-and-paste mechanism. Tn10/IS10was the first bacterial transposon shown to transpose by the cut-and-paste mechanism and as such provided an early model for this mode of transposition (1,2). In cut-and-paste transposition the transposon is first excised from flanking donor DNA by a pair of transposase-catalyzed double-strand breaks at each transposon end after which the excised transposon is inserted into a target site. Host repair of the transposon-target DNA junction completes the transposition process and leaves a characteristic target-site duplication.
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Key words
transposons tn10,tn5
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