Structural Analyses of the Glycolipids in Lipid Rafts.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Lipid rafts are usually isolated from cells or tissues using sucrose gradient ultracentrifugation in the presence of detergents such as Triton X-100 at 4 °C. Although detergents should be removed for further structural characterization following fractionation, these compounds are often difficult to completely remove, especially from the glycolipids. In this chapter, we describe a novel method for the fast and convenient removal of detergents from lipid raft glycolipids following fraction and describe the application of this method.
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关键词
1,2-dichloroethane,Detergent removal,Lipid raft,Mass spectrometry,Membrane microdomain
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