Increased intracellular survival of Salmonella Typhimurium ST313 in HIV‐1‐infected primary human macrophages is not associated with Salmonella hijacking the HIV compartment

Background Non‐typhoidal Salmonella (NTS) causes a severe invasive syndrome (iNTS disease) described in HIV‐positive adults. The impact of HIV‐1 on Salmonella pathogenesis and the molecular basis for the differences between these bacteria and classical diarrhoeal S . Typhimurium remains unclear. Results Here, we show that iNTS‐associated S . Typhimurium Sequence Type 313 (ST313) bacteria show greater intracellular survival in primary human macrophages, compared with a ‘classical’ diarrhoeal S . Typhimurium ST19 isolate. The increased intracellular survival phenotype of ST313 is more pronounced in HIV‐infected macrophages. We explored the possibility that the bacteria take advantage of the HIV‐associated viral‐containing compartments created in human macrophages that have low pH. Confocal fluorescence microscopy and focussed ion beam‐scanning electron microscopy tomography showed that Salmonella did not co‐localise extensively with HIV‐positive compartments. Conclusion The capacity of ST313 bacteria to survive better than ST19 bacteria within primary human macrophages is enhanced in cells pre‐infected with HIV‐1. Our results indicate that the ST313 bacteria do not directly benefit from the niche created by the virus in HIV‐1‐infected macrophages, and that they might take advantage from a more globally modified host cell. Significance A better understanding of the interplay between HIV‐1 and Salmonella is important not only for these bacteria but also for other opportunistic pathogens.