MAPK domain inhibition: Validation of the anti-angiogenic effects of curcumin from Curcuma longa in NDEA model of liver carcinoma in Wistar rats
Research Square (Research Square)(2023)
摘要
Abstract Objectives This study employed MAPK domain inhibition as an anti-angiogenic scaffold and further validate the anti-angiogenic effects of the lead phytochemicals, curcumin from ethanolic extract of curcuma longa (EECL) in N-nitrosodiethylamine (NDEA) model of liver cancer in Wistar rats. Methods One hundred and twenty Wistar rats comprising of sixty male and female rats were randomly selected into twelve groups (n = 5): group A (100 mg/kg NDEA + 200 mg/kg EECL), group B (100 mg/kg NDEA + 400 mg/kg EECL), group C (100 mg/kg NDEA + 600 mg/kg EECL), group D (100 mg/kg NDEA + 200 mg/kg pure curcumin), group E (100 mg/kg NDEA + 100 mg/kg sylibon 140), group F (100 mg/kg NDEA), group G (200 mg/kg pure curcumin), group H (100 mg/kg DMSO), group I (200 mg/kg EECL), group J (400 mg/kg EECL), group K (600 mg/kg EECL), group L (control) at the end of 42 days of the experiment period. The lead phytochemicals, curcumin from EECL were isolated and subjected to Gas Chromatography-Mass Spectrometry for characterization. The anti-angiogenic potentials of the curcumin isolates were validated through molecular docking and the expression of antiangiogenic related mRNA. Results The binding of Co-crystallized, curcumin and cis-sesquisabinene hydrate, to the binding site led to the conformation with binding energies of -15.15 kcal/mol, -7.212 kcal/mol, and − 6.361 kcal/mol respectively. Treatment with 200 mg/kg and 400 mg/kg significantly (p < 0.05) downregulated the expression of MAPK and Vascular endothelial growth factor mRNAs in the hepatocyte tumour, while the Alpha Fero Protein and Interleukin-10 mRNA was significantly (p < 0.05) upregulated. Conclusion Ethanolic extract of Curcumin longa possessed anti-angiogenic and anti-proliferating prospective against MAPK domain inhibition.
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关键词
curcuma longa,curcumin,liver carcinoma,anti-angiogenic
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