VDJServer – a web-accessible analysis portal for immune repertoire sequencing analysis

Abstract VDJServer is a comprehensive, web-accessible system for analysis of immune repertoire sequencing data. VDJServer provides a complete analysis workflow from pre-processing of sequence reads, to V(D)J assignment, to repertoire characterization and comparison. Recent enhancements in VDJServer include: --Automatic parallelization of analysis tools to handle very large data sets running on a high-performance supercomputer--Import and export subject and sample metadata. User-defined sample groups allows for sophisticated group analysis and comparison.--Extensive analysis functionality such as gene segment usage, CDR3 patterns, clonality, diversity measures, somatic mutation patterns, B cell lineage trees, and quantification of selection pressure. Analysis is performed for both samples and sample groups.--Interactive charting of analysis data provides exploratory visualization for ad-hoc comparison of samples and sample groups. Charts can be downloaded as image files for use in presentations and publications. All analysis data can be downloaded in standard TSV format for use with external tools.--Novel process workflow metadata that is automatically captured by VDJServer. Hiding the complexities of command line tools and their parameters, yet providing complete transparency of the analysis workflow for reproducibility. VDJServer allows users to upload antigen receptor repertoire sequences and execute a customizable workflow for all steps in the analysis. Data and analysis results can be privately shared with other users for collaborative projects. VDJServer is funded by the NIAID and is freely available.