Synthesis, Chemical, Morphological and Thermal Characterization Yeast Immobilized Bacterial Cellulose and It Use in Color Removal

Abstract Bacterial cellulose is a biomaterial and can be used for many applications such as medical products and food ingredients with its superior properties such as being pure, high crystallinity and durability. It can also be used as an as an immobilization support. It is a good and practical support for yeast immobilization. The present study aimed to immobilize yeast on lyophilized bacterial cellulose produced by Gluconacetobacter xylinus B759 in cheese whey medium as a cheap and natural culture medium. Optimum conditions such as cultivation time, carbon source, temperature, pH and nitrogen sources for bacterial cellulose production in cheese whey medium were determined. The highest bacterial cellulose yield obtained under optimized conditions was 13.18 g/L. Saccharomyces cerevisiae cells were immobilized on purified and lyophilized 0.5 cm pure bacterial cellulose. Bacterial cellulose and yeast-immobilized bacterial cellulose structures were characterized by different instrumental techniques. Furthermore, Reactive Blue 171 dye decolorization ability of immobilized yeast was tested. The structure of bacterial cellulose showed very suitable morphology and chemical structure for different biotechnological applications. The use of a cheap and natural medium is very important in terms of production conditions, as it is an environmentally friendly application in terms of biotechnology.