CHAPTER 14: Defense mechanisms mediated by chitin in rice-blast interactions

HomeMolecular Plant PathologyGenome-Enabled Analysis of Plant-Pathogen InteractionsCHAPTER 14: Defense mechanisms mediated by chitin in rice-blast interactions PreviousNext CHAPTER 14: Defense mechanisms mediated by chitin in rice-blast interactionsYoko Nishizawa, Susumu Mochizuki, Ken-ichiro Saitoh, Kyutaro Kishimoto, Yusuke Kouzai, and Eiichi MinamiYoko NishizawaSearch for more papers by this author, Susumu MochizukiSearch for more papers by this author, Ken-ichiro SaitohSearch for more papers by this author, Kyutaro KishimotoSearch for more papers by this author, Yusuke KouzaiSearch for more papers by this author, and Eiichi MinamiSearch for more papers by this authorAffiliationsAuthors and Affiliations Published Online:2 Aug 2017https://doi.org/10.1094/9780890544983.014AboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Abstract Chitin is widely distributed in fungal cell walls, and its fragments act as potent elicitors in several plant systems. Indeed, pretreatment of rice seedlings with the chitin elicitor results in increased resistance to rice blast. Our histochemical and reverse genetic analyses suggest that the rice blast fungus possesses a mechanism to avoid generation of the chitin elicitor in the first-invaded cell, but none of the genes encoding chitin-binding domains that were tested was responsible for the mechanism. The release of the chitin elicitor during the infection process is thus limited, and the chitin elicitor induces basal resistance, which is nonspecific but not strong enough to avoid infection completely. Therefore, we manipulated cellular responses upon sensing the chitin to evoke an HR-like response by modifying CEBiP, a receptor for the chitin elicitor, with the intracellular region of XA21. Rice plants constitutively expressing the chimeric receptor displayed enhanced resistance to rice blast. In future studies using molecular breeding for disease resistance, a combination approach in which both the chimeric receptor and chitinase are overexpressed simultaneously, or using another receptor-like protein kinase that induces more effective HR, would be helpful. DetailsFiguresLiterature CitedRelated Genome-Enabled Analysis of Plant-Pathogen InteractionsISBN:978-0-89054-498-3 Metrics Pages: 121-129 InformationPDF download