Lemzeiet, a traditional algerian fermented food: first characterization of lactic acid bacteria flora
HAL (Le Centre pour la Communication Scientifique Directe)(2014)
Abstract
Algerian couscous named Lemzeiet is manufactured from fermented wheat and characterized
by a brown color and a particular flavors especially appreciated by consumers. Historically
performed in underground silos (Matmour) during several years, the fermentation of wheat is
now generally carried out in plastic jerrycans or drums during around one year with or
without addition of vinegar at the beginning of the fermentation. Most of the cereal- based
fermented foods involve lactic acid fermentation including Lemzeiet. The aim of this work
was to determine the dynamic and the diversity of lactic acid bacteria populations over two
years, in the presence or absence of vinegar. Culture dependent methods were used and 147
isolates were selected based on phenotypic characteristics. 70 were retained for molecular
characterization. They were identified by coupling different molecular methods (16S
rRNA/pheS/rpoA genes sequencing and specific PCR) and the clonal diversity was
investigated with randomly amplified polymorphic DNA (RAPD) and pulse-field gel
electrophoresis analysis (PFGE). The population of lactic acid bacteria evolved during the
period of fermentation. It was dominated by Lactobacillus and Pediococcus species in the
wheat fermented without vinegar, and by Enterococcus and Pediococcus species in the
presence of vinegar. We also observed a high level of intra-species diversity. Analysis of
aroma compounds by head space trap-gas chromatography-mass spectrometry is under
progress to determine the impact of the lactic acid bacteria populations on the final aroma
profile of products. These results give a first insight in lactic acid bacteria population diversity
in Lemzeiet and will contribute to a better control of the fermentation process.
MoreTranslated text
Key words
lactic acid bacteria
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined