Ezh2过表达在 mcf －7获得性耐药中的作用

Objective To explore the effect of EZH2 overexpression on acquired drug resistance in MCF-7 cells.Methods The relative expressions of EZH2 mRNA and protein of MCF-7 and MCF-7/ADR were detected respectively by real time fluorescence quantitative PCR ( RTFQPCR)and Western -blot.After EZH2 shRNA and EZH2 shRNA-scramble plasmid vectors with reporter gene EGFP were transfected into MCF -7/ADR cells,the stable cell line was screened by G4l8.RTFQPCR was used to validate whether the EZH 2 mRNA in EZH2 shRNA group was supressed.The differences of the effect of adriamycin on cell proliferation were evaluated among EZH2 shRNA goup,EZH2 shRNA-scramble group and negative control groups using WST-1 assays. Results The EZH2 mRNA and protein expression levels in MCF-7/ADR cells were 2.52 ±1.523 and 1.58 ± 0.58 folds of those in MCF-7cells.The differences were significant(P<0.05).After the EZH2 shRNA plasmid was stably expressed in MCF-7/ADR cells,the expressions of EZH2 mRNA were decreased by 84%(P<0.05) and the cell proliferation was decreased by 25%after adriamycin(P<0.05),However there were no significant changes of cell proliferation in EZH2 shRNA-scramble group and negative control groups after adriamycin .Con-clusion EZH2 was overexpressed in MCF-7 /ADR cells compared with MCF-7 cells.Silencing EZH2 may ef-fectively reverse the resistance of MCF-7/ADR cells to adriamycin.