G-baToN: a versatile cell-cell interaction reporter system
bioRxiv (Cold Spring Harbor Laboratory)(2020)
Abstract
Cell-cell interactions influence all aspects of development, homeostasis, and disease. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon fluorescent protein transfer driven by nanobody-mediated recognition to enable sensitive and specific labeling of cells after cell-cell interactions. A suite of orthogonal baToN tools enables reciprocal cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher order cell-cell interactions across a wide range of cell types.
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Key words
interaction,g-baton,cell-cell
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