An improved high-oleic line created by chemical mutagen of 'Huayu 40'.

By using chemical mutagenesis, ‘Huayu 40’, a normal-oleic（NO） large-seed high yielding peanut cultivar with wide adaptability was transformed into mutants with desirable high-oleic（HO） quality traits, comparable productivity and adaptability. Of the 3 chemical treatments, viz. 5 mM ethyl methane sulfonate（EMS）, 25 mM EMS and 15 mM sodium azide（NaN 3 ）, only NaN 3 produced HO M 2 mutant plants. High oleate phenotype was initially identified by near infrared spectroscopy（NIRS） screening, and was further confirmed by gas spectrometry（GC） analysis. Sequence analysis showed that HO plant had a 448 G＞A mutation in FAD2 A and a 441 4 42 insA mutation in FAD2 B, respectively. Although the direct use of high yielding and stress resistant cultivars/lines to induce quality mutations might be a judicious choice to accelerate breeding, our results showed that chemical mutagenesis could quickly create HO peanut materials and expand the genetic bases of HO peanuts.