Development and Validation of a Recombinant Envelop Glycoprotein Based Indirect Enzyme-Linked Immunosorbent Assay to Detect the Antibody to REV
Agricultural Sciences in China(2010)
摘要
Based on the antigenic analysis of Reticuloendotheliosis virus (REV) envelop glycoprotein (env) protein, an alternative indirect enzyme-linked immunosorbent assay (ELISA) for detection of REV antibody was developed using a truncated env protein of REV produced in Escherichia coli. This assay was validated by comparison with an indirect immunofluorescence assay (IFA) and a REV-based ELISA. The diagnostic sensitivity (DSN), specificity (DSP) and accuracy of the env indirect-ELISA (ienv-ELISA) were 93.7, 93.3 and 93.6% compared with IFA on 1 380 field serum samples, and 84.8, 95.2 and 91.7% compared with the REV-based ELISA on 96 field sera, respectively. Cross-reactivity assay showed that this assay was REV-specific. Repeatability test revealed that the coefficients of variation of positive sera within and between runs were less than 15%. This method is simpler to produce and perform, time-saving and suitable for large scale survey of REV antibody at low cost.
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关键词
antibody,immunosorbent assay,glycoprotein,enzyme-linked
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