Determination of Benzopyrene in Mainstream and Sidestream Smoke

Inder order to determine Benzopyrene (BaP) in Mainstream and Sidestream Smoke, a Solid phase extraction (SPE) cartridge was used to isolate the BaP fraction from the total particulate matter of mainstream cigarette smoke and sidestream cigarette smoke and the BaP were measured by RP-HPLC with ultraviolet detection. The result showed that the recovery rate of BaP extracted is 94.5%, compared with the relative standard of 6.95%. The yields of BaP in mainstream smoke for Virginia cigarette are higher than those for blended cigarettes. The yields of BaP in sidestream smoke are much higher than those in mainstream smoke. The proposed method involves an solid phase extract and HPLC-UV analysis procedure. With this method, the BaP yields in mainstream and sidestream cigarette smoke can be measured fast, easily and precisely using readily available apparatus and instruments.