Antibody Bank: the Synthetic Cell Repertoire for Immunotherapy

Despite promising achievement, current immunotherapy is impeded by disease heterogeneity, cellular plasticity and treatment induced evolution. Here, we proposed a novel artificial bionic immune cell system based on synthetic-receptor library and genetic circuits. In this system, a pre-established population of genetically engineered lymphocyte repertoire was developed, and a particular antigen can activate only its specific counter cell, enable that specific cell to survival and multiply under specific artificial environment, destroying particular antigens. Two synthetic patterns were postulate based on CAR receptors and synNotch receptors, in which the antibody library took place of the antigen-recognition region. An inhibited expression of the inducible caspase-9 suicide gene is coupled with the activation of the synthetic receptor, allowing the lymphocyte repertoire to co-evolve with the dynamic expression of the antigens. In theory, if the diversity of lymphocyte repertoire is sufficient, we can use this artificial system for the treatment of any disease of mankind.