ВЛИЯНИЕ УДАЛЕНИЯ КОРНЕЙ НА СОДЕРЖАНИЕ ЦИТОКИНИНОВ В КЛЕТКАХ АПЕКСА ПОБЕГА АРАБИДОПСИСА

We studied the dependence of cytokinins accumulation in the shoot apexes of Arabidopsis plants on the delivery of these hormones from the roots. For the estimation of cytokinins content in the cells the method of immunohystochemical localization was used using antibodies against zeatin riboside. To prevent washing of cytokinins during the process of dehydration we used the method of differential conjugation of free cytokinins bases and their ribosides. Root cutting has been shown to weaken the immunostaining of zeatin in the cells of the shoot apical meristem thereby supporting the hypothesis about dependence of cytokinins accumulation in these cells on the hormone delivery from the roots. We detected a decline in the level of cytokinins in the cells of the shoot apex as affected by protonophor indicating important role of the process of secondary active transport through the cell membrane for the maintenance of cytokinins level in the cells of the shoot apex.