纤溶酶原激活物抑制因子-1对肝星状细胞转化生长因子β1、透明质酸、基质金属蛋白酶抑制因子-1的影响

：Objective To study the effect of plasminogen activator inhibitortype-1 ( PAI-1 ) on the protein expression of transforming growth factor-beta 1 ( TGFβ1 ), hyaluronic acid ( HA ) and tissue inhibitor ofmetalloproteinase-1 (TIMP-1) of hepatic stellate cells(HSC). Methods Methyl thiazolyltetrazolium (MTT)was performed to detect the effect of PAI-1 on the proliferation of LX-2,and to determine the perfect intervention concentration of PAI-1. After incubation withPAI-1 in LX-2 culture solution for 12 h,24 h,48 h,the protein expression of TGFβ1 ,TIMP-1 and HA was observed with enzyme linked immunosorbentassay (ELISA). Results A492 in the negative control was 0. 473 ± 0. 353, and 1. 249 ± 0.440, 1. 636 ± 0. 315,1.283 ± 0. 413,0. 938 ±0. 263 and 0. 303 ±0. 125 when theconcentration of PAI-1 was 5,10,20,40 and 80 μg/L,respectively,aconcentration of 10 μg/L of PAI-1 showed thestrongest stimulation remarkablely ( F = 11. 697, P ＜ 0. 01 ).After incubation with PAI-1 for 12 h,24 h,48 h,the proteinexpression of HA,TGFβ1 and TIMP-1 in the LX-2supernatant significantly increased compared to negative control (F = 1566. 752,235. 632and 67. 359,respectively,P ＜ 0. 01 or ＜ 0. 05 ). Conclusion PAI-1 may influence the development of hepaticfibrosis through promoting the protein expression of HA ,TGFβ1 and TIMP-1 of HSC.