Bioturbation structures of polychaetes in muddy sediments

X-ray 3D computed microtomography (μCT) is a non-destructive method that provides highresolution image knowledge by a volume data sets of an inspected object which does not need to be previously modified, i.e., the object inspected does no need to be subjected to a preparation method. Its physical principle is based on the X-rays attenuation when they interact with the material (Machado et al. 2013). In order to obtain μCT image it is necessary to acquire many projections in constant angular steps and these reconstruction is performed with an appropriate algorithm based on filtered back-projection (Fledkamp et al. 1984). The first step to achieve 3D data information is to perform segmentation on 2D μCT images, which can be carried out with different approaches depending on the nature/geometry of the investigated structures.