Automated magnetic-bead-assisted sequential extraction technology for simultaneous detection of Aβ1-42 and Aβ1-40 in cerebrospinal fluid: An advance toward fully automated liquid chromatography-tandem mass spectrometry method

•A robust MBASE UPLC–MS/MS method is firstly established for CSF Aβ1-42 and Aβ1-40.•The novel MBASE UPLC–MS/MS method are comparable to the traditional SPE method.•The novel MBASE technology is more convenient and efficient than traditional SPE.•The half-automated MBASE asasy is promising to be developed to be fully-automated.