Divergences in leaf CO₂ diffusion conductance and water use efficiency of soybean coping with water stress and its interaction with N addition

Drought and nitrogen (N) additions have been shown to affect plant photosynthesis, but the effect of their interaction on CO2 diffusion conductance (i.e., stomatal conductance, g(sc), mesophyll conductance, g(m)) and water use efficiency (WUE) at the leaf level (i.e., instantaneous water use efficiency, WUEins; intrinsic water use efficiency, WUEint) has rarely been reported. Herein, we examined the interactive effects of drought and N addition on g(sc) and g(m) and WUE (i.e., WUEins and WUEint) in soybean (Glycine max (Linn.) Merr.) and analysed the interaction mechanisms mitigating water stress based on leaf anatomy and molecular activity. Drought decreased g(sc,) g(m) and WUEins, but N addition had a positive interactive effect with water stress on leaf physiological traits. The variables g(sc), g(m) and WUEins were significantly increased by the additions of 7.5 and 15.0 g urea m(-2) under moderate water stress (MW) conditions (P < 0.05), but they decreased under the interaction effect of SW and N addition. The decreased T-cw and increased S-m and S-c under the water stress x N addition interaction positively affected g(m), and the increased SS dominated the g(sc) increase. In addition, these positive effects were further enhanced with increased AQP, CA and Rubisco activities and a decrease in the ABA content. Accordingly, moderate soil N additions of 7.5 and 15.0 g urea m(-2) greatly mitigated the negative effects of droughts, especially the MW effects, by enhancing CO2 diffusion conductance and WUE.