Rational Design and Biological Application of Hybrid Fluorophores

Fluorophores are considered powerful tools for not only enabling the visualization of cell structures, substructures, and biological processes, but also making for the quantitative and qualitative measurement of various analytes in living systems. However, most fluorophores do not meet the diverse requirements for biological applications in terms of their photophysical and biological properties. Hybridization is an important strategy in molecular engineering that provides fluorophores with complementarity and multifunctionality. This review summarizes the basic strategies of hybridization with four classes of fluorophores, including xanthene, cyanine, coumarin, and BODIPY with a focus on their structure-property relationship (SPR) and biological applications. This review aims to provide rational hybrid ideas for expanding the reservoir of knowledge regarding fluorophores and promoting the development of newly produced fluorophores for applications in the field of life sciences. In this review, we primarily summarized the hybridizations between four classical fluorophores, namely xanthene, cyanine, coumarin and BODIPY. We provided an overview of the connection modes between conjugated and non-conjugated hybrid fluorophores. Hybrid fluorophores have played an irreplaceable role in the fields of biological detection, diagnosis and treatment.image