Assessment of multi-biomedical efficiency of Andrographis paniculata shoot extracts through in-vitro analysis and major compound identification

The present investigation looked into the various biomedical potentials of Andrographis paniculata shoot extracts. The results showed that the methanol extract (Met-E) of A. paniculata contains more phytochemicals than the acetone and petroleum ether extracts, including alkaloids, saponins, tannins, phenolics, flavonoids, glycosides, terpenoids, phytosterol, steroids, and protein. Accordingly, the Met-E alone showed considerable bactericidal activity (through agar well diffusion method) against the bacterial pathogens namely Shigella dysenteriae, Bacillus cereus, Salmonella typhi, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphlococcus aureus, E. coli, and B. subtilis. This bactericidal activity was found as dose dependent manner, since at 1000 mu g ml concentration, the Met-E showed better antibacterial activity. Similarly, at increased concentration (1000 mu g ml) it showed notable antidiabetic (alpha-amylase inhibition: 74.31% and alpha-glucosidase inhibition: 72.34%), antioxidant (DPPH: 78.24%), and anti-inflammatory (albumin denaturation inhibition: 79.84% and lipoxigenase inhibition: 69.4%) activities. The phytochemical profiling of Met-E was characterized by UV-visible spectrophotometer (UV-vis), Gas Chromatography-Mass Spectrometry (GC/MS), Fourier transform infrared (FTIR), and High Per-formance Liquid Chromatography (HPLC) analyses. The results showed the Met-E contain bioactive compounds such as gallic acid, epicatechin, catechin, naringin, vitexin-2-rhamnoside, taxifolin, kaempferol, hesperidin, myricetin, rutin, quercetin, phloretin, and ursolic acid compounds. While most of these substances have been recognised for their pharmacological application perspective, the biological properties of particular substances must be studied in the future using in-vivo strategies.