Acoustic holograms for homogeneous hyperthermia over several tumor spheroids
2023 IEEE International Ultrasonics Symposium (IUS)(2023)
Abstract
In this work we use 3D printed holographic lenses to enlarge the natural focus of a curved transducer with the aim of producing a uniform region of iso-thermal dose. With this system, we exposed several U87-MG glioma cell spheroids, located inside an IEC tissue mimicking phantom, to a uniform and real-time monitored temperature. Spheroids were exposed with this system until different thermal iso-effective doses (7.5, 30, 60, 120 CEM43) (TIDs) were reached. Also, the effects of ultrasound-delivered hyperthermia on the response of these spheroids were compared with heating using a polymerase chain reaction (PCR) thermal cycler in terms of viability and growth measured on a 10-day schedule. Using acoustic holograms designed to deliver iso-thermal doses, we observe a 6 mm wide uniform region of hyperthermia over the three targets, with temperature differences of less than 3%. In addition, tumor spheroid responses show that ultrasound delivered hyperthermia is more damaging than PCR heating for TIDs ≥ 30 CEM43. This system could help in future in-vitro studies of hyperthermia delivered with ultrasound.
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Key words
acoustic holograms,acoustic lenses,ultrasound hyperthermia,tumour spheroids,thermal dose
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