Synthesis of 3-Phenylserine by a Two-enzyme Cascade System with PLP Cofactor

A two-enzyme cascade system containing omega-transaminase (omega-TA) and L-threonine aldolase (L-ThA) was reported for the synthesis of 3-Phenylserine starting from benzylamine, and PLP was utilized as the only cofactor in these both two enzymes reaction system. Based on the transamination results, benzylamine was optimized as an advantageous amino donor as confirmed by MD simulation results. This cascade reaction system could not only facilitate the in situ removal of the co-product benzaldehyde, enhancing the economic viability of the reaction, but also establish a novel pathway for synthesizing high-value phenyl-serine derivatives. In our study, nearly 95 % of benzylamine was converted, yielding over 54 % of 3-Phenylserine under the optimized conditions cascade reaction. A novel cascade system was first proposed for the synthesis of 3-Phenylserine with transaminase and threonine aldolase. This cascade reaction system can not only facilitate the in situ removal of the co-product benzaldehyde, enhancing the economic viability of the reaction, but also the PLP is utilized as the only cofactor in these reaction system.image