Enrichment and distribution of 3,6-dichlorocarbazole in red crucian carp (Carassius auratus) and its hepatotoxicity

Polyhalogenated carbazoles (PHCZs) are a class of organohalogen compounds where the hydrogen atom on the carbazole ring is replaced by a halogen atom. In recent years, PHCZs have drawn increasing concern due to their persistence, dioxin-like toxicity, bioaccumulation, potential ecological hazards and widespread occurrence in the environment. Current research on the enrichment and depuration of PHCZs in biological tissues and organs is insufficient, and the liver toxicity is unclear. Herein, to understand the enrichment and elimination of 3,6-DCCZ in fish tissues and organs as well as the hepatotoxicity, we exposed the red crucian carp to 20 and 100 mu g/L of 3,6-DCCZ for 20 days followed by a depuration period of 10 days. The 3,6-DCCZ enrichment in each organ tissue was classified from high to low: brain > liver, intestine, gill > muscle. For depuration, 3,6-DCCZ was quickly excreted in the various organs of the red crucian carp; however, the liver depuration was slow, with the concentration of 3,6-DCCZ was maintained at 0.25-0.35 mu g/g. 3,6-DCCZ exposure at both tested concentrations induced oxidative stress in red crucian carp, causing lipid peroxidation and DNA damage, as well as some histopathological changes in the liver, such as cell vacuolization, nucleus pyknosis, nucleus pleomorphism, no nucleus areas. Additionally, the 3,6-DCCZ exposure at higher concentration (100 mu g/L) caused more serious damage and abnormal lipid metabolism in the red crucian carp liver.