Lipid Production of Schizochytrium sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate

Marine oleaginous thraustochytrids have attracted increasing attention for their great potential in producing high-value active metabolites using various industrial and agricultural waste. Food waste containing abundant nutrients is considered as an excellent feedstock for microbial fermentation. In this study, a thraustochytrid strain Schizochytrium sp. HBW10 was isolated from a water column in Bohai Bay in Northern China for the first time. Further lipid production characteristics of S. sp. HBW10 were investigated utilizing sulfuric acid hydrolysate of food waste (FWH) from two different restaurants (FWH1 and FWH2) with the initial pH value adjusted by NaOH or NaHCO3. Results showed that the highest concentration of total fatty acids (TFAs) was observed in FWH2 medium with the 50% content level on the fifth day, reaching up to 0.34 g/L. A higher initial pH promoted the growth and saturated fatty acid (SFA) accumulation of S. sp. HBW10, achieving nearly 100% of the sum of saturated and monounsaturated fatty acids (SMUFAs) in TFAs with initial pH7 and pH8 in FWH1 medium. This work demonstrates a possible way for lipid production by thraustochytrids using food waste hydrolysate with a higher initial pH (pH7 similar to pH8) adjusted by NaHCO3.