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山桐子灰斑病病原菌鉴定及防治药剂筛选

WANG Li,WU Yuekai, HU Yiwen, HUANG Hao,HU Kai,YANG Zaihua

Agrochemicals(2023)

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Abstract
[目的]明确山桐子重要病害——灰斑病的病原菌,筛选出山桐子灰斑病的高效防治药剂.[方法]通过对分离物的致病性接种测试、形态特征观察以及ITS、TEF-1α和TUB序列的分析进行鉴定,并采用菌丝生长速率法测定了22种杀菌剂对病原菌的抑制效果.[结果]分离培养所得菌株STZHBB-1通过人工接种试验产生的症状与田间自然感病症状一致.经过形态学和分子生物学鉴定将山桐子灰斑病的病原菌鉴定为交链格孢(Alternaria alternata).室内防治药剂筛选结果表明,对病原菌菌丝生长抑制效果最好的是复合芽孢杆菌、多粘类芽孢杆菌,EC50值分别为0.1964、0.8527 mg/L.[结论]可为山桐子灰斑病的诊断及防治提供理论依据.
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Key words
Idesia polycarpa,gray spot disease,pathogen identification,Alternaria alternata,fungicide screening
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