Assessment of bioactive chemicals in wastewater effluents and surface waters using in vitro bioassays in the Nakdong River basin, Korea

Organic micropollutants present in effluents of wastewater treatment plants (WWTPs) can negatively affect the quality of receiving waters or drinking water sources. The present work monitored the concentration of bioactive chemicals using a battery of in vitro bioassays in 14 WWTP effluents, 2 effluent-dominant streams, and 5 river waters in the Nakdong River basin, Korea, for a two-year period. The WWTP effluents showed AR/ERα/TRβ (androgen/estrogen/thyroid hormone) activities at a few to tens ng/L, PAH/PPARγ/p53 (polycyclic-aromatic-hydrocarbon/lipid metabolism/genotoxicity) activities at hundreds ng/L, and PXR/Nrf2 (xenobiotic metabolism/oxidative stress) activities at tens to hundreds μg/L as bioanalytical equivalent concentrations. The concentration level and type of bioactivities were statistically not affected by the source, season, or treatment processes of WWTPs for most endpoints. The effluent-dominant streams showed similar levels of AR/ERα/PAH/PXR/Nrf2 activities compared to the upstream WWTP effluents. The river waters showed lower levels of AR/ERα activities (by factors of 6 or 7) but had only slightly lower PAH/PXR/Nrf2 activities (within factors of 2) than the WWTP effluents when compared based on median concentration. Cytotoxicity was below the quantification limit (0.3 μg/L) in most effluent and river samples. For ERα/PAH/PXR/Nrf2, the median bioactivity levels of the river waters were higher than at least one of the effect-based trigger (EBT) values proposed in the literature. Further monitoring work and reliable/realistic EBT derivation are needed to determine possible ecological risks posed by the observed bioactivities.