Herbivore insect small RNA effector suppress plant defense by cross-kingdom gene silencing
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Herbivore insects deploy salivary effectors to manipulate the defense of their host plants, however, whether insect small RNAs (sRNAs) act as effectors to regulate plant-insect interaction is currently unclear. Here, we report that a microRNA (miR29-b) from the saliva of phloem-feeding insects can transfer into the host plant phloem and fine-tune the host defense. The salivary gland’s abundant miR29-b was produced by insect Dicer 1 and insect salivary exosome is involved in its transferring and releasing into the host plant. Insect miR29-b effector hijacks plant Argonaute 1 to silence host defense gene Bcl-2-associated athanogene 4 ( BAG4 ). Silencing of BAG4 suppressed the expression of phenylalanine ammonia-lyase and the accumulation of salicylic acid (SA), therefore negatively regulating host defense against herbivore insects. miR29-b is highly conserved in Hemiptera, Coleoptera, Hymenoptera, Orthoptera, and Blattaria insects and also targets the BAG4 gene. Notably, BAG4 orthologs exist in a wide range of plant species and may as the target of insect miR29-b. Our work provides new insight into the intriguing defense and counter-defense between herbivores and plants.
Teaser Phloem-feeding insects produce and transfer small RNA into the host plants to fine-tune plant basal defense by cross-kingdom gene silencing.
### Competing Interest Statement
The authors have declared no competing interest.
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关键词
Plant-insect interactions,RNA Interference,Viral RNA Silencing
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