Aedes aegypti microbiome composition covaries with the density of Wolbachia infection

Jane Pascar, Henry Middleton,Steve Dorus

MICROBIOME(2023)

引用 0|浏览4
暂无评分
摘要
Background Wolbachia is a widespread bacterial endosymbiont that can inhibit vector competency when stably transinfected into the mosquito, Aedes aegypti , a primary vector of the dengue virus (DENV) and other arboviruses. Although a complete mechanistic understanding of pathogen blocking is lacking, it is likely to involve host immunity induction and resource competition between Wolbachia and DENV, both of which may be impacted by microbiome composition. The potential impact of Wolbachia transinfection on host fitness is also of importance given the widespread release of mosquitos infected with the Drosophila melanogaster strain of Wolbachia ( w Mel) in wild populations. Here, population-level genomic data from Ae. aegypti was surveyed to establish the relationship between the density of w Mel infection and the composition of the host microbiome. Results Analysis of genomic data from 172 Ae. aegypti females across six populations resulted in an expanded and quantitatively refined, species-level characterization of the bacterial, archaeal, and fungal microbiome. This included 844 species of bacteria across 23 phyla, of which 54 species were found to be ubiquitous microbiome members across these populations. The density of w Mel infection was highly variable between individuals and negatively correlated with microbiome diversity. Network analyses revealed w Mel as a hub comprised solely of negative interactions with other bacterial species. This contrasted with the large and highly interconnected network of other microbiome species that may represent members of the midgut microbiome community in this population. Conclusion Our bioinformatic survey provided a species-level characterization of Ae. aegypti microbiome composition and variation. w Mel load varied substantially across populations and individuals and, importantly, w Mel was a major hub of a negative interactions across the microbiome. These interactions may be an inherent consequence of heightened pathogen blocking in densely infected individuals or, alternatively, may result from antagonistic Wolbachia -incompatible bacteria that could impede the efficacy of w Mel as a biological control agent in future applications. The relationship between w Mel infection variation and the microbiome warrants further investigation in the context of developing w Mel as a multivalent control agent against other arboviruses. AACNEMfgWJ36enPNiFi-k- Video Abstract
更多
查看译文
关键词
Dengue virus,Core microbiome,Vector competency,Mosquito,Pathogen blocking
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要