Flow cytometry for the assessment and monitoring of aberrant intraepithelial lymphocytes in non-responsive celiac disease and non-celiac enteropathies
DIGESTIVE AND LIVER DISEASE(2024)
摘要
Background: Few data are available on flow cytometry (FC) for monitoring intraepithelial lymphocytes (IELs) in refractory celiac disease (RCD), non-responsive celiac disease (NRCD), and non-celiac enteropathies (NCEs). AIMS.1) To investigate the significance of monitoring IELs immunophenotype with FC in patients with NRCD, RCD and NCEs; 2) to evaluate FC concordance with immunohistochemistry (IHC) and gamma-TCR clonality analysis. Methods: Patients investigated between January-2012 and February-2023 were divided into two groups: 1)confirmed RCD or NRCD being investigated for persistent symptoms and suspected complications of celiac disease (CD); 2)NCEs lacking clinical/histological response. Clinical/molecular features and outcomes were retrospectively collected and analysed according to presence/absence of aberrant IELs on FC (cut-off >= 20 % CD103+sCD3-CD8-iCD3+ IELs). Results: 52 patients (18 RCD,21 NRCD,13 NCEs; 38F, 55 +/- 13 years; median follow-up 30 months, IQR 2-58) underwent 100 FC IELs determinations. 22/52 had >= 2 FC determinations and IEL phenotype remained unchanged over time in all them (kappa=1.00). Aberrant IEL phenotype in CD was associated with increased mortality (HR 4.2, 95 % CI 1.5-11.9, p < 0.01). No patients with NCEs had an aberrant IEL phenotype at FC, although 3/13 developed lymphoma and 4/13 died. Concordance of FC was fair with both IHC (kappa=0.40) and gamma-TCR clonality analysis (kappa=0.22). Conclusion: FC is accurate for assessing and monitoring IEL phenotype and providing important prognostic information in celiac patients. Further study is needed on its role in NCEs. (c) 2023 The Author(s). Published by Elsevier Ltd on behalf of Editrice Gastroenterologica Italiana S.r.l. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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关键词
Celiac disease,Flow cytometry,Intraepithelial lymphocytes,Mortality,Villous atrophy
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