Cytotoxic and anti-inflammatory constituents from roots of Hypericum beanii and the antitumor potential under the view of cancer-related inflammation

Hypericum beanii, a traditional folk medicine plant, has been employed in the treatment of various inflammationrelated diseases and has demonstrated promising potential as an herbal remedy for cancer. In this study, we isolated 29 compounds from the roots of H. beanii. We evaluated their cytotoxic effects on five human cancer cell lines, which revealed that the ethanol extract, along with compounds 4 and 14, exhibited significant cytotoxic activity. Additionally, we assessed their anti-inflammatory properties by measuring the inhibition of nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages. Our findings showed that the ethanol extract (IC50 = 7.41 +/- 0.38 mu g/mL), compound 4 (IC50 = 7.82 +/- 0.42 mu M), and compound 14 (IC50 = 3.05 +/- 0.06 mu M) displayed substantial anti-inflammatory activity. ELISA assays and qPCR analysis revealed that compounds 4 and 14 may exert their anti-inflammatory and antitumor effects by inhibiting the expression of iNOS, TNF-alpha, IL-1 beta, and IL-6 mRNA, shedding light on their role in cancer-related inflammation.