Research Progress on Detection Methods of Lytic Polysaccharide Monooxygenase Activities

Lytic polysaccharide monooxygenases (LPMOs) are a class of copper ion-dependent monooxygenase, as a sort of industrial enzymes known for their catalytic degradation of recalcitrant polysaccharides, such as cellulose and chitin. LPMOs can improve the accessibility and hydrolysis efficiency of glycoside hydrolases to polysaccharide substrates by oxidizing and breaking the glycoside bonds in the crystalline region, showing strong application potential in the conversion of industrial biomass. The detection methods of LPMOs activities with high sensitivity and high throughput are of great significance in the mining, engineering transformation and industrial application of new LPMOs. Based on the catalytic mechanism of LPMOs and the diversity of their oxidative products, the common detection methods of LPMOs activities were summarized and compared in this review, mainly focusing on the application of chromatography-mass spectrometry, colorimetry and fluorescence probe labeling and their latest research progress, and the advantages and disadvantages of different methods and their application scope were also compared. Further, the future development trends of detection methods of LPMOs activities were prospected.