Microporous Annealed Particle (MAP) Scaffold Pore Size Influences Mesenchymal Stem Cell Metabolism and Proliferation Without Changing CD73, CD90, and CD105 Expression Over Two Weeks

Scaffold pore architecture is shown to influence stem cell fate through various avenues. It is demonstrated that microporous annealed particle (MAP) microgel diameter can be tuned to control scaffold pore size and, in turn, modulate mesenchymal stem cell (MSC) survivability, proliferation, metabolism, and migration, thereby enhancing bioactivity and guiding future applications of MAP for regenerative medicine. Scaffold pore size is shown to be a bioactive feature and can be tuned in microporous annealed particle scaffolds through microgel diameter. In this manuscript, it demonstrate the impact of porosity on mesenchymal stem cell (MSC) survivability, proliferation, metabolism, and migration. It is believed that the ability to expand MSCs independent of phenotype shift in a 3D format will provide future routes to the manufacturing and study of MSCs.image