Algal or bacterial community: Who can be an effective indicator of the impact of reclaimed water recharge in an urban river.

Reclaimed water has been widely utilized for water resource replenishment, yet little is known regarding its impacts on various microorganisms in the receiving water. To address this knowledge gap, we systematically investigated the responses of bacteria and algae to the recharge of reclaimed water by using the high-throughput sequencing technology in the urban Chaobai River. After the inputs of reclaimed water, lower contents of NO2--N, NH4+-N, and TP were observed in the downstream section compared to that of upstream without reclaimed water, indicating that reclaimed water could improve the water quality of the receiving water. Correspondingly, both bacterial and algal communities showed the decreased network complexity in the downstream section, but many common freshwater bacteria and typical bloom-forming algae were dominant in the downstream, potentially suggesting that algae were more sensitive to the local environmental conditions. More importantly, although nitrogen and phosphorus served as the paramount factors in shaping both bacterial and algal communities, environmental selection contributed more to algal rather than bacterial community, and simultaneously algal variations could further affect bacterial dynamics in the urban river. Overall, these findings revealed distinct characteristics of bacteria and algae in responding to the reclaimed water recharge, highlighting the superiority of algae in indicating environmental changes, especially in monitoring and regulating the replenishment of reclaimed water in urban rivers.